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Quantitative CTC reduction was more closely correlated with respiration in whole cells than INT, but the reverse was true in inverted membrane vesicles. We undertook studies to determine the sites, and degree of coupling for INT and CTC reduction in the aerobic Escherichia coli K respiratory chain using both intact cells and inverted membrane vesicles.
Both tetrazolium salts were reduced to significant degrees under anaerobic conditions, particularly glucose fermentation. Results of experiments with inhibitors of specific electron transport components indicated that both CTC and INT were reduced prior to ubiquinone in the E.